An Exact Correction to the “Cheng-Prusoff” Correction
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 8 (1-4), 533-546
- https://doi.org/10.3109/10799898809049010
Abstract
The use of graphical estimation techniques in pharmacology is well entrenched, yet can sometimes lead to confusion and errors. The widely used Cheng-Prusoff correction for obtaining the inhibition constant Ki from the graphical mid point or ED50 of a displacement or inhibition curve is not exact, contrary to popular belief. We show that under many commonly used experimental conditions, the Cheng-Prusoff rule can lead to a substantial 3- to 6-fold or larger error in the estimate of Ki. Surprisingly, an explicit, relatively simple exact correction rule is available. We derive this rule and show the conditions under which its use is required.Keywords
This publication has 10 references indexed in Scilit:
- [41] LIGAND: A computerized analysis of ligand binding dataMethods in Enzymology, 1983
- Model Testing in Radioligand/Receptor Interaction by Monte Carlo SimulationJournal of Receptor Research, 1983
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980
- The determination of binding parameters when the total and free substrate concentrations are not approximately equalBiochemical Journal, 1979
- Enzyme changes in neonatal skeletal muscle: effect of thyroid deficiencyAmerican Journal of Physiology-Cell Physiology, 1978
- Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reactionBiochemical Pharmacology, 1973
- Mathematics of Hormone-Receptor InteractionAdvances in Experimental Medicine and Biology, 1973
- Mathematical theory of complex ligand-binding systems at equilibrium: Some methods for parameter fittingAnalytical Biochemistry, 1972
- COMPUTER ANALYSIS OF RADIOLIGAND ASSAY AND RADIOIMMUNOASSAY DATAActa Endocrinologica, 1970
- Enzyme and metabolic inhibitorsPublished by Biodiversity Heritage Library ,1963