Continuous Registration of Membrane Input Resistances of Small Plant Cells Using a Double-Pulse Current Clamp Technique for Single-Electrode Impalements

Abstract

To measure the cell input resistance in Elodea leaf cells, a new single-microelectrode method was explored by comparing the results with conventional 2-microelectrode experiments. The new method takes advantage of the difference in the frequency response curves between electrode and cell impedances. By application of electrical stimuli, which contain specific frequency bands, the different impedances can be analyzed separately. To get a distinct separation in the frequency response of cell and electrode, respectively, the electrode capacitance has to be compensated during the impalement. Different time constants of the cell membrane can be accounted for by adjustment of the stimulus length. Both the single- and double-electrode method yield the same results, even if the cell input resistances change considerably during the course of the experiment. This demonstrates the usefulness of the new single-electrode method for continuous measurements of cell membrane resistances, especially in cells so small that the double-electrode method is no longer applicable.