Induction of somatic embryogenesis in leaf-derived callus of Vicia narbonensis L.
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (5), 267-269
- https://doi.org/10.1007/bf00274126
Abstract
A method for the induction of somatic embryogenesis in callus cultures, using explants from mature leaves of Vicia narbonensis L., is described. Callus developed on a solid medium (Murashige and Skoog 1962), which was supplemented with low concentrations of picloram and benzylaminopurine. Subsequent culture was carried out in different liquid media (culture length four months). The gradual reduction of auxin and cytokinin concentrations, and the addition of glutamine and pyridoxal·HCl were favourable. Somatic embryos appeared on solid media without phytohormones.Keywords
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