Multiple Forms of Lysyl-tRNA Synthetase fromEscherichia coli

Abstract

Lysyl-tRNA synthetase was isolated from E. coli. The enzymatic activity elutes as 3 or 4 bands from a hydroxylapatite column. Polyacrylamide gel analysis shows that each of these bands contains more than 1 enzymatically active protein species. The MW of the subunits of these species provide an explanation for the variation in the MW previously reported for this enzyme.