Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO‐12049 in E. coli
- 19 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (1), 36-40
- https://doi.org/10.1016/0014-5793(88)80980-3
Abstract
The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of M r29 966, which was close to the value of the lipase expressed in E. coli.Keywords
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