Vasoactive intestinal peptide: A neurotrophic releasing agent and an astroglial mitogen
- 1 March 1990
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 25 (3), 386-394
- https://doi.org/10.1002/jnr.490250316
Abstract
Vasoactive intestinal peptide (VIP) increases neuronal survival in dissociated spinal cord cultures during a critical period of development. In the present study, two mechanisms contributing to this action of VIP have been observed: (1) VIP was shown to be a secretagogue for neuron survival-promoting activity; and (2) VIP was found to be an astroglial mitogen. A high molecular weight substance (>30 kDa), which increased neuronal survival in tetrodotoxin (TTX)-treated spinal cord cultures, was detected in the medium from nonneuronal cells incubated for 1 hr with 0.1 nM VIP. In addition, 3H-thymidine autoradiography and glial fibrillary acid protein (GFAP) immunocytochemistry were used to show that a 5 day treatment with (VIP) increased astroglial mitosis. This effect was specific for astroglia, as silver grain-positive cells not exhibiting GFAP immunoreactivity did not increase in number after VIP treatment. The dual action of VIP may regulate glial-derived trophic substances that are important for neuronal survival during the course of development.Keywords
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