Genetic control of isozyme variation in Camellia japonica L.

Abstract

Procedures are described for the extraction and electrophoretic separation of enzymes from seed and leaves of the tannin-rich evergreen Camellia Japonica L. Seventeen enzyme systems have been resolved, and of these all but three were polymorphic. Crosses were performed between parents bearing dissimilar allelomorphs in order to discern the genetic control of the resolved enzymes. Based on the segregation ratios observed, 12 of the zones of staining for eight enzyme systems were postulated to be codominantly inherited single-gene traits. Tests of joint segregation for 58 out of 66 possible pairwise combinations for the 12 postulated loci suggested that two pairs of genes are linked: AAT-1 with PGM-3 (r = 0.29 ± 0.03), and 6-PGD-2 with PGM-2 (r = 0.17 ± 0.02).