Lens Permeability Changes in Relation to X-Ray Induced Cataract as Detected by Cell-Mediated Immune Activity

Abstract

The experimental radiation cataract in rabbits served as a research model in an investigation of the relation between cataract progression and lens permeability changes. Slit-lamp examination revealed the first lens opacities 12 weeks following X-irradiation. Sensitization of the cellular immune system of the rabbits as detected by skin tests and lymphocyte stimulation was found to be maximal 8 weeks following X-ray treatment. This sensitization was predominantly directed to low molecular weight lens proteins, i.e., proteins other than intact α-crystallin. The results point to a leakage of low molecular weight lens proteins out of the lens before cataract is clinically detectable.