Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum

Abstract
Seven cell lines of N. tabacum resistant to the herbicide picloram were isolated from cell cultures. In crosses of plants regenerated from 4 cell lines, resistance was inherited as a single dominant Mendelian allele. Plants could not be regenerated from 1 cell line, and expression of resistance proved unstable in 2 others.