Electron transfer to nitrogenase in Klebsiella pneumoniae. nifF gene cloned and the gene product, a flavodoxin, purified
- 1 November 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 231 (3), 743-753
- https://doi.org/10.1042/bj2310743
Abstract
The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain. Characterization of the isolated flavodoxin, which serves as an electron donor to nitrogenase, shows unambiguously that it is the product of the nifF gene.This publication has 34 references indexed in Scilit:
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