Occurrence of 1st division metaphases in human lymphocyte cultures
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 41 (1), 97-100
- https://doi.org/10.1007/bf00278875
Abstract
Human lymphocytes were cultured for 40, 42 and 44h and analysed for the number of 1st and 2nd division metaphases using the BrdU-Giemsa technique. The most suitable time to obtain the majority of cells in their 1st division concomittant with an adequate mitotic index was 42h. It is recommended that laboratories use sister chromatid differential staining to determine the most suitable culture time for the analysis of 1st division metaphases. This will permit accurate comparisons between individuals and laboratories to be made.This publication has 10 references indexed in Scilit:
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