The minimum surface fibrinogen concentration necessary for platelet activation on dimethyldichlorosilane‐coated glass
- 1 March 1991
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 25 (3), 407-420
- https://doi.org/10.1002/jbm.820250311
Abstract
Albumin and fibrinogen were competitively adsorbed onto dimethyldichlorosilane‐coated glass (DDS‐glass) and platelet activation was examined as a function of the surface fibrinogen concentration. The weight ratio of albumin to fibrinogen in the adsorption solution was varied from 10 to 700. Platelet activation was quantitated by the area and circularity of spread platelets. When the DDS‐glass was coated with albumin alone, platelets were only contact adherent and could not spread at all. After competitive adsorption of fibrinogen and albumin, however, platelets were able to spread on the surface. Platelet activation increased linearly as the surface fibrinogen concentration increased up to 0.02 μg/cm2. Platelets were able to activate fully if the surface fibrinogen concentration was 0.02 μg/cm2 or higher, even though the surface was dominated by albumin. It appears that platelets can activate fully as long as only a small fraction (2–15%) of the surface is covered with tightly bound fibrinogen.Keywords
This publication has 41 references indexed in Scilit:
- Identification of plasma proteins adsorbed to hemodialyzers during clinical useJournal of Biomedical Materials Research, 1989
- Transient adsorption of fibrinogen on foreign surfaces: Similar behavior in plasma and whole bloodJournal of Biomedical Materials Research, 1989
- The Fate of Fibrinogen following Adsorption at the Blood‐Biomaterial, InterfaceaAnnals of the New York Academy of Sciences, 1987
- Adsorption to Biomaterials from Protein MixturesPublished by American Chemical Society (ACS) ,1987
- Collagen at interfaces II: Competitive adsorption of collagen against albumin and fibrinogenJournal of Biomedical Materials Research, 1987
- Biomaterials in Artificial OrgansChemical & Engineering News, 1986
- SURFACE‐INDUCED PLATELET ADHESION, AGGREGATION, AND RELEASE*†Annals of the New York Academy of Sciences, 1977
- IN VITRO EVALUATION SYSTEMS: A BIOLOGICAL APPROACHAnnals of the New York Academy of Sciences, 1977
- Bovine Plasma Protein Adsorption onto Radiation-Grafted Hydrogels Based on Hydroxyethyl Methacrylate and N-VinylpyrrolidonePublished by American Chemical Society (ACS) ,1975
- Cell Surface Movements Related to Cell LocomotionPublished by Wiley ,1973