Histamine‐Dependent Allergy Blood Test
- 1 September 1986
- Vol. 41 (7), 526-531
- https://doi.org/10.1111/j.1398-9995.1986.tb00338.x
Abstract
Allergen-mediated histamine release from human leukocytes represents an important model for in vitro studies of allergic reactions. The purpose of this study was to determine whether the measurement of histamine released in allergic patients by radioenzymatic assay following mixing of their blood with common allergens represents a reliable index for diagnosis of atopic allergy. Three categories of allergens were used: 1) house dust and mite; 2) cat and dog dander; 3) trees, gasses and ragweed mixture. The presence of allergy was established by clinical history and intradermal skin testing in the study group of 150 patients. A significant allergen-mediated histamine release ranging from 4 to 65% of the total blood histamine content was observed in 96 % of the patients with skin test sensitivity of ≥ 3+. There was a significant correlation between skin testing and histamine release in terms of the allergens causing the response. Thus, the measurement of histamine by radioenzymatic technique following its release in blood in response to allergen challenge represents a clinically useful in vitro test for the diagnosis of atopic disease.Keywords
This publication has 12 references indexed in Scilit:
- Prevalence and spectrum of asthma in childhood.BMJ, 1983
- An Enzymatic-Isotopic Microassay for Measuring Allergic Release of Histamine from Blood and Mast Cells in vitroInternational Archives of Allergy and Immunology, 1980
- The Role of Basophils in Inflammatory ReactionsJournal of Investigative Dermatology, 1978
- Reappraisal of intracutaneous tests in the diagnosis of reaginic allergyJournal of Allergy and Clinical Immunology, 1978
- Allergic skin testing: A clinical investigationJournal of Allergy and Clinical Immunology, 1977
- Automated histamine analysis for in vitro allergy testingJournal of Allergy and Clinical Immunology, 1977
- Diagnostic tests in ragweed hay fever: A comparison of direct skin tests, IgE antibody measurements, and basophil histamine releaseJournal of Allergy and Clinical Immunology, 1973
- The radioallergosorbent test in the in vitro diagnosis of multiple reaginic allergyJournal of Allergy and Clinical Immunology, 1971
- In vitro Diagnosis of Atopic AllergyInternational Archives of Allergy and Immunology, 1971
- In Vitro Studies of Human Ragweed Allergy: Changes in Cellular and Humoral Activity Associated with Specific Desensitization*JCI Insight, 1966