Stimulation of Respiration and Secretion of Mouse Pancreas in Vitro

Abstract

Mouse pancreas has been incubated in vitro with the parasympathomimetic drugs pilocarpine and Carbachol and with the hormones pancreozymin and secretin. The first three substances stimulated both respiratory rate and rate of enzyme secretion whereas secretin stimulated only the respiratory rate. The time-course of stimulation by pancreozymin was followed. Its effect on the respiratory rate was immediate while the largest secretion of ribonuclease occurred after 30 minutes of incubation. The secretory and respiratory effects of pancreozymin were not decreased by atropine but were slightly lessened by banthine. Large differences were found in the nonstimulated extrusion of ribonuclease in different strains of mice.