A monoclonal antibody that defines an idiotope with two subsites in galactan-binding myeloma proteins.
Open Access
- 1 December 1981
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 154 (6), 1946-1956
- https://doi.org/10.1084/jem.154.6.1946
Abstract
An IgG1 monoclonal antibody HyX24-14 was derived from A/J mice that were immunized with the IgA XRPC24 (X24) galactan binding myeloma protein (GalBMP) of BALB/c origin by the Kohler-Milstein hybridoma technology. HyX24-14 specifically binds some but all GalBMP. Different patterns of binding using a panel of nine Gal BMP were found, depending upon the concentration of antibody and the antigenic target. From molecular models and amino acid sequence data, ti was proposed that the idiotope defined by HyX24-14 had two subsites, each of which appeared to be able to bind independently to the antibody.This publication has 16 references indexed in Scilit:
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