Sulfate reduction by a Desulfovibrio species isolated from sheep rumen.
- 1 September 1974
- journal article
- Vol. 28 (3), 489-97
Abstract
Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. Cell-free extracts show absorption maxima for cytochrome c(3) and desulfoviridin, characteristic of Desulfovibrio. Carbohydrates, as a sole carbon source, will support growth. Lactate supports growth in the presence of sulfate, not in its absence, whereas glucose or pyruvate support growth either in the presence or absence of sulfate. The isolate has a deoxyribonucleic acid base composition of 61.2% guanine plus cytosine, which is similar to that of several other species of Desulfovibrio; however, it differs from previously described species in morphology, motility, and carbon source utilization. Cell-free extracts of this bacterium exhibit adenosine 5'-triphosphate-sulfurylase, adenosine-5'-phosphosulfate-reductase, and hydrogenase activity. After incubation of cell-free extracts with adenine 5'-triphosphate and (35)SO(4) (2-), adenosine-5'-phosphosulfate rather than 3'-phosphoadenosine-5'-phosphosulfate was shown to be labeled, indicating that the pathway of sulfate reduction in this organism is similar to that of other dissimilatory sulfate reducers. This is the first report of a Desulfovibrio sp. isolated from the rumen.This publication has 19 references indexed in Scilit:
- Copper-Molybdenum Interactions with the Sulfate-Reducing System in Rumen MicroorganismsExperimental Biology and Medicine, 1972
- ATP-Sulfurylase From Penicillivm Chbysogenum. I. Purification and CharacterizationPreparative Biochemistry, 1971
- The Evaluation of Media used to Enumerate Sulphate Reducing BacteriaJournal of Applied Bacteriology, 1970
- Effect of dietary sulfur upon the fatty acid production in the rumenBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- Studies on adenosine 5′-phosphosulfate reductase from desulfovibrio desulfuricans and thiobacillus thioparus I. The assay and purificationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- A Sulphate-Reducing Bacterium from the Sheep RumenJournal of General Microbiology, 1960
- THE ATP-DEPENDENT REDUCTION OF SULFATE WITH HYDROGEN IN EXTRACTS OF DESULFOVIBRIO DESULFURICANSProceedings of the National Academy of Sciences, 1959
- A Diagnostic Reaction of Desulphovibrio desulphuricansNature, 1959
- Effect of Volatile Fatty Acids, Sodium and Potassium Bicarbonate in Purified Diets for Ruminants.Experimental Biology and Medicine, 1959
- The reduction of sulphate in the rumen of the sheepBiochemical Journal, 1954