Characterization of Celullases and Related Enzymes by Isoelectric Focusing, Gel Filtration, and Zone Electrophoresis. II. Studies on Stereum sanguinolentum, Fomes annosus, and Chrysosporium lignorium Enzymes.

Abstract

Some properties of the enzymes cellulase, mannanase, xylanase, [beta]-glucosidase, aryl-[beta]-glucosidase, mannosidase, and xylosidase obtained from culture filtrates of the fungi Stereum sanguinolentum, Fomes annosus, and Chrysosporium lignorum have been Investigated by gel filtration, isoelectric focusing and zone electrophoresis. With few exceptions, the isoelectric points of the enzymes were found to be in the pH range 3.6-4.7. The extracellular endoenzymes were found to be of smaller molecular sizes than their corresponding exoenzymes, as previously reported. The relation between the electrophoretic mobilities of 2 proteins could not be predicted even if the isoelectric points and the relation between the molecular sizes were known.