Simplified Spectrophotometric Assay of Serum Glutamic Oxaloacetic Transaminase and Lactic Dehydrogenase

Abstract

This paper deals with an economical, simplified modification of the methods for determining the levels of glutamic oxaloacetic transaminase (S-GOT) and lactic dehydrogenase (S-LD) in plasma (or in serum). A single blank of reduced cozymase (DPN-H) may be used for numerous determinations on test specimens. The modified procedure yields reliable and accurate results, and it is saving of time and reagents. By means of defining a unit of S-LD as the amount that produces a change of 0.01 in the optical density, data for S-LD may be adjusted so that they are numerically comparable with those for S-GOT. Comparison of levels of S-GOT and S-LD in 10 patients with acute infarcts of the myocardium indicated that the activity of both enzymes was greater than normal. Although the patterns of activity of the 2 enzymes were closely correlated, the level of S-LD reached a peak later, and returned to normal later than that for S-GOT.