Helper function of T cells depleted of alloantigen-reactive lymphocytes by filtration through irradiated F1 hybrid recipients. I. Failure to collaborate with allogeneic B cells in a secondary response to sheep erythrocytes measured in vivo.
Open Access
- 1 September 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 144 (3), 617-626
- https://doi.org/10.1084/jem.144.3.617
Abstract
Helper T [thymus-derived] cells were obtained by injecting heavily irradiated semiallogeneic mice with lymph node cells from H-2-incompatible parental strain mice primed with sheep erythrocytes (SRC) 2 mo. before. Thoracic duct lymphocytes collected from the recipients 18-40 h later (nearly all of which were .theta.-positive and of donor origin) were toatally and specifically unresponsive against host-type determinants in mixed-lymphocyte culture. The filtered cells were transferred to irradiated semiallogeneic mice together with SRC and anti-.theta.-serum-treated (B [bone marrow-derived] cells from SRC-primed syngeneic, semiallogeneic or allogeneic mice. When antibody-forming cells were measured in the spleen 5-9 days later, effective IgM [immunoglobulin M] and IgG collaborative responses were observed with syngeneic and semiallogeneic B cells but not with allogeneic cells. The failure to obtain collaboration with the allogeneic B cells was probably not due to inhibition of the B cells by the T cells or vice versa.This publication has 18 references indexed in Scilit:
- Effect of recent antigen priming on adoptive immune responses. III. Antigen-induced selective recruitment of subsets of recirculating lymphocytes reactive to H-2 determinants.The Journal of Experimental Medicine, 1976
- T Cell Function in Bone Marrow Chimeras; Absence of Host‐Reactive T Cells and Cooperation of Helper T Cells across Allogeneic BarriersImmunological Reviews, 1976
- Collaboration of allogeneic T and B lymphocytes in the primary antibody response to sheep erythrocytes in vitro.The Journal of Experimental Medicine, 1975
- GENETIC CONTROL OF THE ANTIBODY RESPONSE TO POLY-L(TYR,GLU)-POLY-D,L-ALA--POLY-L-LYS IN C3H↔CWB TETRAPARENTAL MICEThe Journal of Experimental Medicine, 1974
- Genetic control of the immune response to (T,G)-A--L in C3H ↔ C57 tetraparental miceCellular Immunology, 1974
- Cell‐mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouseEuropean Journal of Immunology, 1974
- THE CLONAL NATURE OF ALLO‐ANTIGEN‐SENSITIVE SMALL LYMPHOCYTES IN THE RECIRCULATING POOL OF NORMAL RATSImmunology & Cell Biology, 1974
- CELL INTERACTIONS BETWEEN HISTOINCOMPATIBLE T AND B LYMPHOCYTESThe Journal of Experimental Medicine, 1973
- Specific Unresponsiveness of Recirculating Lymphocytes after Exposure to Histocompatibility Antigen in F1 Hybrid RatsNature New Biology, 1971
- SOME FURTHER DATA ON CYTOTOXIC ISOANTIBODIES IN THE MOUSE*Annals of the New York Academy of Sciences, 1962