Three replication origins in Sulfolobus species: Synchronous initiation of chromosome replication and asynchronous termination
- 23 April 2004
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (18), 7046-7051
- https://doi.org/10.1073/pnas.0400656101
Abstract
Chromosome replication origins were mapped in vivo in the two hyperthermophilic archaea, Sulfolobus acidocaldarius and Sulfolobus solfataricus, by using microarray-based marker frequency analysis. Bidirectional replication was found to be initiated in near synchrony from three separate sites in both organisms. Two of the three replication origins in each species were located in the vicinity of a cdc6/orc1 replication initiation gene, whereas no known replication-associated gene could be identified near the third origin in either organism. In contrast to initiation, replication termination occurred asynchronously, such that certain replication forks continued to progress for >40 min after the others had terminated. In each species, all replication forks advanced at similar DNA polymerization rates; this was found to be an order of magnitude below that displayed by Escherichia coli and thus closer to eukaryotic elongation rates. In S. acidocaldarius, a region containing short regularly spaced repeats was found to hybridize aberrantly, as compared to the rest of the chromosome, raising the possibility of a centromere-like function.Keywords
This publication has 18 references indexed in Scilit:
- Identification of Two Origins of Replication in the Single Chromosome of the Archaeon Sulfolobus solfataricusCell, 2004
- An Archaeal Chromosomal Autonomously Replicating Sequence Element from an Extreme Halophile,Halobacteriumsp. Strain NRC-1Journal of Bacteriology, 2003
- Genus-Specific Protein Binding to the Large Clusters of DNA Repeats (Short Regularly Spaced Repeats) Present in Sulfolobus GenomesJournal of Bacteriology, 2003
- Replication Dynamics of the Yeast GenomeScience, 2001
- Cell cycle regulation in the hyperthermophilic crenarchaeon Sulfolobus acidocaldariusMolecular Microbiology, 2001
- Analysis of topoisomerase function in bacterial replication fork movement: Use of DNA microarraysProceedings of the National Academy of Sciences, 2000
- Altered patterns of cellular growth, morphology, replication and division in conditional-lethal mutants of the thermophilic archaeon Sulfolobus acidocaldariusMicrobiology, 2000
- The Sulfolobus-?Caldariella? group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerasesArchiv für Mikrobiologie, 1980
- Phylogenetic structure of the prokaryotic domain: The primary kingdomsProceedings of the National Academy of Sciences, 1977
- Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperatureArchiv für Mikrobiologie, 1972