A mucinous-like carcinoma-associated antigen (MCA) in the tissue and blood of patients with primary breast cancer
- 1 February 1989
Abstract
The monoclonal antibody (MAb) b12 raised against human breast cancer cell lines was found to identify an epitope of a mucinous‐like carcinoma associated antigen (MCA) that is strongly represented on breast tumor cells. The b12 MAb was used to develop an enzyme immunoassay (ElA) kit. MCA levels were measured with the EIA method in the cytosol of both breast cancer and normal breast tissue as well as in the blood of 147 patients with primary breast cancer and 92 healthy subjects. MCA cytosol levels were significantly higher in carcinoma than in normal breast tissue cytosol samples. Higher MCA levels were found in the cytosol of tumor without lymph nodal involvement. The 95th percentile of the MCA value distribution in the healthy control group (11.0 U/ml) was chosen as negative/positive cut‐off level. The overall positivity rate in breast cancer group was 26.5% with MCA showing a trend toward higher levels in patients with more advanced disease. Significantly higher levels were found in patients with a higher number of positive lymph nodes.This publication has 19 references indexed in Scilit:
- Monoclonal antibodies against antigens on breast cancer cellsCellular and Molecular Life Sciences, 1985
- Tumor markers: Value and limitations in the management of cancer patientsCancer Treatment Reviews, 1985
- Biological markers for breast carcinomaCancer, 1984
- Differential Reactivity of a Novel Monoclonal Antibody (DF3) with Human Malignant versus Benign Breast TumorsHybridoma, 1984
- Membrane 126-kilodalton phosphoglycoprotein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells.Proceedings of the National Academy of Sciences, 1983
- A monoclonal antibody (B72.3) defines patterns of distribution of a novel tumor‐associated antigen in human mammary carcinoma cell populationsInternational Journal of Cancer, 1982
- A spectrum of monoclonal antibodies reactive with human mammary tumor cells.Proceedings of the National Academy of Sciences, 1981
- The monitoring role of plasma CEA alone and in association with other tumor markers in colorectal and mammary carcinomaCancer, 1978
- Clinical correlation between CEA and breast cancerCancer, 1978
- Carcinoembryonic antigen in breast cancer patients: Serum levels and disease progressCancer, 1974