Preparation of Human Chorionic "Growth Hormone-Prolactin."

Abstract
Summary A simple procedure for partial purification of human chorionic “growth hor-mone-prolactin” is described utilizing DEAE-cellulose chromatography and Sephadex G-75 filtration. The preparation has a high degree of homogeneity. However, small amounts of serum protein contaminants were detected. The electrophoretic mobility in starch gel of the isolated hormone, utilizing a modified discontinuous buffer system was comparable to that of the “native” hormone in a freshly prepared crude placental extract.