Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
Open Access
- 29 May 2009
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 4 (5), e5730
- https://doi.org/10.1371/journal.pone.0005730
Abstract
Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.Keywords
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