HETEROGENEOUS OXYGEN PARTIAL-PRESSURE AND PH DISTRIBUTION IN C3H MOUSE MAMMARY ADENOCARCINOMA

Abstract

Severe disturbances in microcirculation during advanced phases of tumor growth lead to restrictions of convective and diffusive transport. An inhomogeneous distribution of transport conditions develops, resulting in insufficient and heterogeneous substrate supply and an inadequate drainage of wastes. Polarographic measurements of the local tissue oxygen tension (PO2) using gold microelectrodes reveal that very low PO2 values are prevalent in C3H mouse mammary carcinomas. The tissue PO2 frequency distributions are shifted to low PO2 values and limited in variability. The mean PO2 value is 7 mm Hg. The median is 4 mm Hg, the modal class being 0-5 mm Hg. Within different microareas of the same tumor, pronounced heterogeneities exist. Due to an elevated rate of lactic acid production and its subsequent inadequate removal, a severe tissue acidosis is evidenced in malignant tumors. For C3H mouse mammary carcinomas, most of the measured pH values are in the range of 6.4-7.1, the modal class being 6.7-6.8 (mean pH, 6.73; median pH, 6.75). Within different microareas of the same tumor, clear heterogeneities in the pH distribution do occur. Very low pH values (5.8-6.3) were observed in large ulcerated tumors. In extensively necrotic areas, pH values even higher than the arterial pH could be detected.