Microanalysis of proteins and peptides. II. Fluorescent labeling of proteins and a plasma membrane using cycloheptaamylose-dansyl chloride complex.

Abstract

A complex between cycloheptaamylose and dansyl chloride was prepared and the product (CDC) was applied to the fluorescent labeling of proteins in aqueous media. Excellent degree of labeling was achieved without using any organic solvent. CDC was found to be moderately soluble in the presence of excess cycloheptaamylose and highly soluble in the presence of urea in aqueous solution. CDC-cycloheptaamylose system was successfully applied to the fluorescent labeling of rat liver plasma membrane. CDC in urea solution provided dansylation method for proteins in a homogeneous system. Rapid dansylation was observed in all the methods described.