In vitro and in vivo Transferrable β‐Lactam Resistance Due to a New Plasmid‐Mediated Oxyiminocephalosporinase from a Clinical Isolate of Proteus mirabilis
- 1 February 1991
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 35 (2), 87-97
- https://doi.org/10.1111/j.1348-0421.1991.tb01537.x
Abstract
A new plasmid-mediated beta-lactamase (FPM-1) with an isoelectric point of 7.2 and a molecular weight of 26,000 was found in a cefuroxime-resistant clinical isolate of Proteus mirabilis strain 6003. FPM-1 can be classified as a type I oxyimino-cephalosporinase on the basis of its substrate specificity and inhibition pattern by clavulanic acid etc., and its conferred resistance on both the strain and transconjugants against most oxyme-type cephalosporins as well as the older ones but not against cefamycins and a few exceptional oxyme-type cephalosporins such as ceftizoxime, ceftazidime and cefixime. In a murine systemic infection model, only these FPM-1-stable drugs exhibited protective activity against the FPM-1-producing P. mirabilis 6003 similar to that against a nonproducing derivative strain. The FPM-1-mediated cefuroxime resistance in P. mirabilis 6003 was transferred to co-infected Escherichia coli 7004 at frequencies between 3.8 x 10(-3) and 4.0 x 10(-2) in a murine ascending urinary tract infection model. In the same infection model due to the FPM-1-producing E. coli transconjugant, FPM-1-stable cefixime was significantly more effective than FPM-1-labile cefteram pivoxil, although both drugs had similar therapeutic effect against its FPM-1-nonproducing counterpart strain.Keywords
This publication has 20 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Extended-spectrum beta-lactamasesAntimicrobial Agents and Chemotherapy, 1989
- Plasmid-Determined Beta-LactamasesPublished by Springer Nature ,1989
- Novel plasmid-mediated beta-lactamase from Escherichia coli that inactivates oxyimino-cephalosporinsAntimicrobial Agents and Chemotherapy, 1988
- Chromosomal Cephalosporinases Responsible for Multiple Resistance to Newer β-Lactam AntibioticsAnnual Review of Microbiology, 1987
- Conjugative transfer of staphylococcal antibiotic resistance markers in the absence of detectable plasmid DNAAntimicrobial Agents and Chemotherapy, 1986
- In-vitro andin-vivo activities of antimicrobial agents againstAcinetobacter calcoaceticusJournal of Antimicrobial Chemotherapy, 1985
- Purification and Some Properties of a Cephalosporinase from Proteus vulgarisAntimicrobial Agents and Chemotherapy, 1981
- The Use of Analytical Isoelectric Focusing for Detection and Identification of -LactamasesJournal of General Microbiology, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970