Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (2), 741-752
- https://doi.org/10.1093/nar/15.2.741
Abstract
The cDNA for mouse thymidinekinase (TK) was isolated from a cDNA library in lambda-gt11 and sequenced. It was used as a probe to follow the time course of TKmRNA expression in growth stimulated mouse fibroblasts. Linked to the HSV-TK promoter the cDNA was able to transform LTK−-cells to the TK− phenotype. The transformed cells expressed the TKmRNA and enzyme activity in a growth dependent fashion suggesting that the regulatory element is localized on the cDNA.Keywords
This publication has 24 references indexed in Scilit:
- Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.Molecular and Cellular Biology, 1986
- Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutationsJournal of Virology, 1983
- Regulation of thymidine kinase enzyme level in serum-stimulated mouse 3T6 fibroblasts*1Experimental Cell Research, 1982
- Cell cycle-dependent regulation of thymidine kinase activity introduced into mouse LMTK- cells by DNA and chromatin-mediated gene transfer.Proceedings of the National Academy of Sciences, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Human deoxythymidine kinase. II: Substrate specificity and kinetic behavior of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemiaBiochemistry, 1976
- Thymidine kinase, DNA synthesis and cancerMolecular and Cellular Biochemistry, 1976
- Thymidine Kinase Isozymes of Normal and Virus-infected CellsCold Spring Harbor Symposia on Quantitative Biology, 1974