Purification of Cathepsin B from Oocytes and Mature Eggs of the Sea Urchin Brissopsis lyrifera by Means of Gel Filtration.
- 1 January 1964
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 18 (9), 2044-2050
- https://doi.org/10.3891/acta.chem.scand.18-2044
Abstract
Cathepsin B from a homogenate fraction of Brissopsis oocytes has been purified 350 times by means of one gel filtration on Sephadex G-100. The chromatograms from oocytes and mature eggs differ in respect to highmolecular material, which, in the chromatogram from mature eggs is strongly reduced. The molecular weight of cathepsin B is estimated at a value of 50,000[long dash] 60,000.Keywords
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