Synthesis of Heat Shock Proteins in Rat Liver After Ischemia and Hyperthermia

Abstract

Ischemia of rat liver is followed by recovery or cell death. Since heat shock proteins may be essential to cell survival under stress, we determined levels of heat shock proteins in liver after different periods of blood deprivation and correlated the results with cellular recovery. Cell–free synthesis by poly (A+)–mRNA and polysomes revealed 70 and 89 kd proteins which appear similar to proteins produced by the liver of rats with amphetamine–induced hyperthermia. The 70 and 89 kd proteins increased in the liver of rats which recovered from ischemia.