Characterization of HETEs and related conjugated dienes by UV spectroscopy
- 1 April 1988
- Vol. 23 (4), 340-344
- https://doi.org/10.1007/bf02537345
Abstract
Three distinct pairs of HETEs can be distinguished on the basis of their UV spectra. We used hydroxy-linoleates (hydroxy-octadeca-cis-trans-dienoates) as a base for comparisons; both the 9- and 13-hydroxy isomers have identical chromophores with λmax near 234 nm. The presence of a double bond three carbons removed from the conjugated diene (the chromophore of 9- and 11-HETE) causes a shift in the observed λmax to near 235 nm. A double bond β to the chromophore (5- and 15-HETE) gives a further shift of 1.5 nm, giving a λmax between 236–236.5 nm. With double bonds in both these positions (8- and 12-HETE), the λmax is observed near 237 nm. It is apparent that the exact λmax of thecis-trans diene chromophore is influenced in a consistent way by the adjacent methylene interruptedcis double bonds.This publication has 5 references indexed in Scilit:
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