CAPILLARY PERMEABILITY TO INTRAVENOUSLY ADMINISTERED GELATINE

Abstract

By plasmapheresis and gelatine injs. in 6 dogs, serum proteins were reduced to 0.9-3.5 gs. % and gelatine concs. varied from 1.5-3.0 gs. %, Following severe pinching of the bowel wall of 4 dogs, gut loops were coiled in a beaker outside the abdomen, and fluid exuding from the surface was collected. Samples of blood and fluid were taken at intervals from 0.5-7 hrs. following the gelatine inj.; bowel manipulation being repeated frequently. The samples were analyzed for serum proteins, gelatine and N. P. N. The ratio of plasma proteins in the fluid to that in the serum was approx. 1.0 in all cases, but the corresponding ratio for gelatine ranged from 0.5-0.65 in all cases. In 2 dogs, a lesser degree of capillary injury, produced by mild manipulation and salting of the bowel surface, yielded lower values for the fluid/serum ratios. These ranged from 0.7-0.85 for plasma proteins and from 0.4-0.47 for gelatine. The "relative leakage ratios" [image] were 0.58, 0.58, and 0.55, which were nearly identical with the avg. of 0.59 (range 0.47 to 0.64) obtained from the more severely injured capillaries of the 1st expts. Only 0.5 to 0.6 as much gelatine leaks through injured capillaries as do plasma proteins.