Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
- 8 July 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (8), 641-643
- https://doi.org/10.1038/nmeth1070
Abstract
The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.Keywords
This publication has 15 references indexed in Scilit:
- Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light outputProtein Engineering, Design and Selection, 2006
- Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumorsProtein Engineering, Design and Selection, 2006
- Self-illuminating quantum dot conjugates for in vivo imagingNature Biotechnology, 2006
- Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in VivoMolecular Therapy, 2005
- Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivoJournal of Biomedical Optics, 2005
- Real-time imaging of TRAIL-induced apoptosis of glioma tumors in vivoOncogene, 2003
- Optical imaging of Renilla luciferase reporter gene expression in living miceProceedings of the National Academy of Sciences, 2001
- A clearer vision for in vivo imagingNature Biotechnology, 2001
- Light-emitters involved in the luminescence of coelenterazineLuminescence, 2000
- Semi-synthetic aequorin. An improved tool for the measurement of calcium ion concentrationBiochemical Journal, 1988