Inhibition of Balbiani ring RNA synthesis at the initiation level.
- 1 March 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (3), 947-950
- https://doi.org/10.1073/pnas.72.3.947
Abstract
The nucleoside analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, a selective inhibitor of heterogeneous chromosomal RNA synthesis in salivary gland cells of the midge Chironomus tentans, blocks the initiation of transcription in Balbiani rings 1 and 2. The analogue seems to be without appreciable effect on the elongation of growing RNA chains and allows finished molecules to be detached from the chromosomal sites. The RNA chain growth rate, based on measurements of the time required for completion of synthesis of Balbiani ring 1 and 2 RNA molecules after addition of the inhibitor, was estimated to be around 25 nucleotides per second at 18 degrees.Keywords
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