Factors Affecting In vitro DNA Ejection of the Lactobacillus lactis Bacteriophage LL-H
- 28 February 1982
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 59 (1), 173-175
- https://doi.org/10.1099/0022-1317-59-1-173
Abstract
Summary Tris(hydroxymethyl)aminomethane (tris) inactivates Lactobacillus lactis bacteriophage LL-H in vitro. The inactivation is caused by DNA ejection. This effect occurs in tris-HCl buffer only. Both pH and temperature affect the sensitivity of the phage to the tris treatment. The divalent cation Mg2+ prevented the inactivating effect of tris at concentrations about 103-fold lower than for the monovalent cation K+.Keywords
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