Evaluation of a “Kit” Method for the Measurement of Total Serum Globulins

Abstract

An evaluation of a commercial kit for the determination of total serum globulin concentration is presented. The method involves the reaction of glyoxylic acid with serum globulins and is standardized with a solution containing human gamma globulin and albumin. The precision of the method is 2.77% and gamma globulin added to serum is quantitatively recovered. Close correlations between this kit and the accepted protein fractionation reference procedures of electrophoresis and salt fractionation were found.