THE USE OF GRADED COLLODION MEMBRANES FOR THE CONCENTRATION OF A BACTERIAL ENZYME CAPABLE OF DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUS
Open Access
- 1 August 1935
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 62 (2), 271-279
- https://doi.org/10.1084/jem.62.2.271
Abstract
1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mµ but is held back by pores having a diameter of 8.2 mµ. 2. When enzyme solutions are filtered to dryness through membranes of such porosity as to hold back the active principle, and when proper precautions are taken to prevent or minimize adsorption, the enzyme can be completely recovered in solution by immersing the membrane in distilled water or physiological salt solution. 3. These results are discussed with reference to the dimensions of the enzyme particle, and to the purification obtained in the course of ultrafiltration. 4. A practical method is described for the concentration and purification of the crude enzyme preparation by the use of graded collodion membranes.This publication has 2 references indexed in Scilit:
- FACTORS AFFECTING THE YIELD OF SPECIFIC ENZYME IN CULTURES OF THE BACILLUS DECOMPOSING THE CAPSULAR POLYSACCHARIDE OF TYPE III PNEUMOCOCCUSThe Journal of Experimental Medicine, 1932
- DECOMPOSITION OF THE CAPSULAR POLYSACCHARIDE OF PNEUMOCOCCUS TYPE III BY A BACTERIAL ENZYMEThe Journal of Experimental Medicine, 1931