The Effect of Temperature and Relative Humidity on the Longevity of the Conidia of Helminthosporium oryzae

Abstract
The effect of temp. on the longevity of the conidia of H. oryzae was investigated by storing small samples of conidia in screw cap vials at temps. ranging from 2-31 [degree]C. Viability was measured periodically by determining the % of conidia capable of germinating within 4 hrs. on potato-dextrose agar. After 100 days'' storage, 81% of the conidia stored at 2[degree]C were viable, while 6% of those stored at 31 [degree]C were viable. In expts. in which 5 temps. ranging from 10-31 [degree]C were employed and rel. humidities of 20, 45, 70, and 95% were provided by H2SO4 solns., the conidia retained their viability best when stored under cool dry conditions. The importance of rel. humidity was shown by the fact that at 20% rel. humidity the conidia survived for 6 months with little loss of viability even at 31[degree]C, whereas at 95% they failed to germinate after 1 month at 31[degree].