Decreased atrial natriuretic peptide binding in renal medulla in rats with chronic heart failure.
- 1 January 1988
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 62 (1), 155-161
- https://doi.org/10.1161/01.res.62.1.155
Abstract
The relations between atrial natriuretic peptide (ANP) binding sites in the renal medulla, plasma ANP concentration, and ventricular dysfunction have been studied in rats 4 weeks after myocardial infarction induced by left coronary artery ligation. Plasma ANP concentration was measured by radioimmunoassay, and quantitation of receptors was performed by computerized in vitro autoradiography with 125I-labeled alpha-rat ANP (1-28) as the radioligand. When compared with controls, rats with myocardial infarction had markedly elevated plasma immunoreactive ANP concentrations (462 +/- 82 versus 124 +/- pg/ml, p less than 0.01) and reduced densities of ANP binding in the inner renal medulla (2.93 +/- 0.19 versus 3.53 +/- 0.22 fmol/mg protein, p less than 0.01). Extensive myocardial infarction was associated with a significant decrease in receptor numbers in the inner medulla (33.6 +/- 5.7 versus 95.6 +/- 9.6 fmol/mg protein, p less than 0.01) without significantly altering the affinity constant (1.76 +/- 0.51 versus 1.03 +/- 0.15 x 10(9) M-1, p greater than 0.05). Right ventricular weight increased in proportion to infarct size (r = 0.71, p less than 0.01), and both were correlated with plasma immunoreactive ANP levels (r = 0.74, p less than 0.01 and r = 0.75, p less than 0.01, respectively). Binding densities in the inner medulla of rats with infarcts were negatively correlated with right ventricular weight, plasma immunoreactive ANP concentrations, and also with infarct size (r = -0.92, p less than 0.001; r = -0.78, p less than 0.001; r = -0.77, p less than 0.01, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)This publication has 25 references indexed in Scilit:
- Atrial natriuretic factor in normal subjects and heart failure patients. Plasma levels and renal, hormonal, and hemodynamic responses to peptide infusion.JCI Insight, 1986
- NATURE OF ATRIAL NATRIURETIC PEPTIDE IN PLASMA FROM PATIENTS WITH CONGESTIVE HEART FAILUREThe Lancet, 1986
- Platelet binding sites for atrial natriuretic factor in humans. Characterization and effects of sodium intake.Hypertension, 1986
- Atrial Natriuretic Peptide Elevation in Congestive Heart Failure in the HumanScience, 1986
- Plasma levels of immunoreactive atrial natriuretic factor in healthy subjects and in patients with edema.JCI Insight, 1985
- Atrial natriuretic factor increases cyclic GMP and inhibits cyclic AMP in rat renal papillary collecting tubule cells in cultureBiochemical and Biophysical Research Communications, 1985
- Atrial natriuretic factor—a circulating hormone stimulated by volume loadingNature, 1985
- Bioactive Cardiac Substances: Potent Vasorelaxant Activity in Mammalian AtriaScience, 1983
- A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in ratsLife Sciences, 1981
- Hormonal regulation of peptide receptors and target cell responsesNature, 1979