Equine Herpesviruses: Antigenic Relationships and Deoxyribonucleic Acid Densities
- 1 October 1973
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 8 (4), 621-627
- https://doi.org/10.1128/iai.8.4.621-627.1973
Abstract
Equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm3 were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. Of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruses were serologically similar to one another; each of them grew more quickly than did any of the other 10 isolates although the mean plaque sizes formed a series of gradations with no clear hiatus which would permit the unequivocal delineation of the abortion viruses from the slowly growing strains. The 10 slowly growing isolates showed antigenic heterogeneity even though complement was present; the neutralizing capacity of an antiserum against the heterologous strains was, in most instances, markedly less than against the homologous strains, the range of the 50% endpoints being much greater than that observed among the equine abortion viruses, or among isolates of herpes simplex type 1. There was no cross neutralization between the equine abortion viruses and any of the 10 slowly growing isolates. An extra band of deoxyribonucleic acid, at 1.723 to 1.725 g/cm3, was present in two of the slowly growing strains when originally grown in rabbit cells, but was no longer present after passage in cat cells. This band occupied the same position as one reported in the hamster-passaged strain of equine abortion virus, and had a density similar to that of the equine genital herpesvirus. Although the taxonomic demarcation of the equine abortion viruses and the slowly growing herpesviruses from one another is still open to question, they can be conveniently labeled equine herpesviruses 1 and 2, respectively; the genital virus would be termed equine herpesvirus 3.Keywords
This publication has 20 references indexed in Scilit:
- Studies of human cytomegalovirus strain variations by kinetic neutralization testsArchiv für die gesamte Virusforschung, 1972
- Serologic differentiation of human cytomegalovirus strains using rabbit hyperimmune seraArchiv für die gesamte Virusforschung, 1971
- 2. Persistence of Equine Herpesviruses in Experimentally Infected Horses and the Experimental Induction of AbortionAustralian Veterinary Journal, 1970
- 1. Isolation and Characterisation of Equine Rhinopneumontis Virus and Other Equine Herpesviruses from HorsesAustralian Veterinary Journal, 1970
- Cytomegalovirus infections in newborn infantsThe Journal of Pediatrics, 1969
- AN EQUINE GENITAL INFECTION RESEMBLING COITAL EXANTHEMA ASSOCIATED WITH A VIRUSAustralian Veterinary Journal, 1969
- Density difference of DNA of human herpes simplex viruses, types I and IIVirology, 1968
- Properties of the nucleic acids from some herpes group virusesVirology, 1964
- Equine herpes virusesVirology, 1963
- Serologic differentiation of viruses responsible for cytomegalic inclusion diseaseVirology, 1960