The type I activin receptor ActRIB is required for egg cylinder organization and gastrulation in the mouse
Open Access
- 15 March 1998
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 12 (6), 844-857
- https://doi.org/10.1101/gad.12.6.844
Abstract
ActRIB is a type I transmembrane serine/threonine kinase receptor that has been shown to form heteromeric complexes with the type II activin receptors to mediate activin signal. To investigate the function of ActRIB in mammalian development, we generated ActRIB-deficient ES cell lines and mice by gene targeting. Analysis of the ActRIB −/− embryos showed that the epiblast and the extraembryonic ectoderm were disorganized, resulting in disruption and developmental arrest of the egg cylinder before gastrulation. To assess the function of ActRIB in mesoderm formation and gastrulation, chimera analysis was conducted. We found that ActRIB −/− ES cells injected into wild-type blastocysts were able to contribute to the mesoderm in chimeric embryos, suggesting that ActRIB is not required for mesoderm formation. Primitive streak formation, however, was impaired in chimeras when ActRIB −/− cells contributed highly to the epiblast. Further, chimeras generated by injection of wild-type ES cells intoActRIB −/− blastocysts formed relatively normal extraembryonic tissues, but the embryo proper developed poorly probably resulting from severe gastrulation defect. These results provide genetic evidence that ActRIB functions in both epiblast and extraembryonic cells to mediate signals that are required for egg cylinder organization and gastrulation.Keywords
This publication has 53 references indexed in Scilit:
- Clonal Analysis of Cell Fate During Gastrulation and Early Neurulation in the MousePublished by Wiley ,2007
- Intracellular signalling: The Mad way to do itCurrent Biology, 1996
- The Tumor Suppressor Gene Brca1 Is Required for Embryonic Cellular Proliferation in the MouseCell, 1996
- TGFβ Signaling: Receptors, Transducers, and Mad ProteinsCell, 1996
- Functional analysis of activins during mammalian developmentNature, 1995
- The TGF-beta superfamily: new members, new receptors, and new genetic tests of function in different organisms.Genes & Development, 1994
- Targeted mutation of the DNA methyltransferase gene results in embryonic lethalityCell, 1992
- Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.Genes & Development, 1991
- Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structuresCell, 1990
- Expression pattern of the mouse T gene and its role in mesoderm formationNature, 1990