Radioimmunoassay of Porcine FSH2

Abstract

A radioimmunoassay for porcine FSH has been developed and validated. The iodinated antigen contained a contaminant that cross reacted with porcine serum. This contaminant was removed by rechromatography of the labeled antigen on a column containing a dowex resin that was equilibrated with porcine serum. Porcine PRL, ACTH and GH did not compete in the assay. On a mass basis, 125 to 150 times more porcine LH was required for equivalent displacement of ¹³¹I PFSH from the antibody. The biologic-radioimmunologic ratios of several crude pituitary extracts ranged from .45 to 1.33. The minimal sensitivity of the RIA was 2.5 ng PFSH LER 1132 (66-67) and the FSH in 500 ul or less of porcine serum or plasma could be measured accurately and precisely. The RIA was employed to determine the concentration of FSH in the serum of female swine during different experimental states. Copyright © 1974. American Society of Animal Science . Copyright 1974 by American Society of Animal Science.