High-Level Resistance to Ceftazidime Conferred by a Novel Enzyme, CTX-M-32, Derived from CTX-M-1 through a Single Asp240-Gly Substitution
Open Access
- 1 June 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (6), 2308-2313
- https://doi.org/10.1128/aac.48.6.2308-2313.2004
Abstract
A clinical strain of Escherichia coli isolated from pleural liquid with high levels of resistance to cefotaxime, ceftazidime, and aztreonam harbors a novel CTX-M gene (blaCTX-M-32) whose amino acid sequence differs from that of CTX-M-1 by a single Asp240-Gly substitution. Moreover, by site-directed mutagenesis we demonstrated that this replacement is a key event in ceftazidime hydrolysisKeywords
This publication has 26 references indexed in Scilit:
- Effect of D240G substitution in a novel ESBL CTX-M-27Journal of Antimicrobial Chemotherapy, 2003
- ColE1-Like Plasmid pIP843 of Klebsiella pneumoniae Encoding Extended-Spectrum β-Lactamase CTX-M-17Antimicrobial Agents and Chemotherapy, 2002
- CTX-M-Type Extended-Spectrum β-Lactamase That Hydrolyzes Ceftazidime through a Single Amino Acid Substitution in the Omega LoopAntimicrobial Agents and Chemotherapy, 2001
- Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance ThreatClinical Microbiology Reviews, 2001
- Novel Cefotaximase (CTX-M-16) with Increased Catalytic Efficiency Due to Substitution Asp-240→GlyAntimicrobial Agents and Chemotherapy, 2001
- Nucleotide Sequence and Characterization of a Novel Cefotaxime-Hydrolyzing β-Lactamase (CTX-M-10) Isolated in SpainAntimicrobial Agents and Chemotherapy, 2001
- Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum β-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytocaBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Site-directed mutagenesis by overlap extension using the polymerase chain reactionGene, 1989
- Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9Gene, 1986
- The Use of Analytical Isoelectric Focusing for Detection and Identification of -LactamasesJournal of General Microbiology, 1975