Ion transport in liver mitochondria from normal and thyroxine-treated rats
- 1 December 1980
- journal article
- research article
- Published by Springer Nature in Journal of Bioenergetics and Biomembranes
- Vol. 12 (5-6), 379-393
- https://doi.org/10.1007/bf00748766
Abstract
Liver mitochondria isolated from rats 24 h after a single subcutaneous injection of 8 mg thyroxine per kilogram body weight were compared with those isolated from control rats that received injections of isotonic saline at the same time. The mitochondria isolated from the thyroxine-treated rats show higher rates of energy-dependent K+ and phosphate accumulation than those from control animals. It was also found that mitochondria from the hormone-treated animals required a larger addition of Ca2+/mg mitochondrial protein in order to uncouple oxidative phosphorylation, and showed smaller tendency to swellin vitro under energizing conditions. The data obtained on ion movements support previous observations that the stimulation of the basal rate of mitochondrial respiration by thyroxine is associated with an increase in the transmembrane protonic electrochemical potential difference, and indicate thatin vivo the hormone raises the intramitochondrial concentration of K+ and phosphate.This publication has 48 references indexed in Scilit:
- Competitive inhibition of valinomycin‐induced K+‐transport by Mg2+‐ions in liver mitochondriaFEBS Letters, 1977
- Effect of Mg2+ depletion of mitochondria on their permeability to K+: The mechanism by which ionophore A23187 increases K+ permeabilityBiochemical and Biophysical Research Communications, 1977
- Role of mitochondria in control of calcium content of liver slicesNature, 1976
- The uptake and extrusion of monovalent cations by isolated heart mitochondriaMolecular and Cellular Biochemistry, 1976
- The effect of thyroidectomy on the subcellular Mg distribution in rat liverLife Sciences, 1975
- Similarity of effects of iodine and thyroxine upon rat liver mitochondriaBiochemical and Biophysical Research Communications, 1962
- A modified single solution method for the determination of phosphate in natural watersAnalytica Chimica Acta, 1962
- Osmotic reversal of mitochondrial swellingBiochimica et Biophysica Acta, 1961
- Some observations on the photometric estimation of mitochondrial volumeBiochimica et Biophysica Acta, 1958
- Mitochondrial oxidative phosphorylation in a magnesium-free mediumBiochimica et Biophysica Acta, 1956