Estimation by radioimmunoassay of VH determinants (Aa1) associated with rabbit T lymphocytes
- 1 February 1977
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 7 (2), 104-110
- https://doi.org/10.1002/eji.1830070210
Abstract
A radioimmunoassay with specificity for the al allotype determinant of rabbit immunoglobulin (Ig) heavy chains was used for the quantitation of VH in detergent extracts of lymph node cells from a1a1/b4b4 rabbits. B cell-derived Ig in the same extracts was estimated with a radioimmunoassay specific for L-chain. About 2 × 105 molecules/cell was found by both assays when assuming a molecular weight of 1.5 × 105 dalton. Extracts prepared from lymphocytes depleted of B cells (surface Ig+ cells) were also found to contain corresponding amounts of a1 and L-chain, with values varying from about 103 to 104 molecules/cell. The estimates were not influenced by the presence of inhibitors of proteolytic enzymes. Supernatants, obtained after keeping the lymphocytes in culture for 16 h, were analyzed by the same methods. Again, matching amounts of a1 and L-chain were found in supernatants from nonfractionated as well as from B cell-depleted populations. The results obtained indicate that T cells do not carry or produce an excess of a1 compared to L-chain, as would have been expected if the T cell antigen receptor carried the same variable region as the Ig heavy chain while being otherwise composed of nonimmunoglobulin-like structures.This publication has 29 references indexed in Scilit:
- VH and L‐chain allotype determinants of rabbit IgG and IgA estimated by a semi‐automatic, modified Farr‐type radioimmunoassayEuropean Journal of Immunology, 1977
- Idiotypic analysis of lymphocytes in vitro. II. Genetic control of T-helper cell responsiveness to anti-idiotypic antibody.The Journal of Experimental Medicine, 1976
- Facilitation or attenuation of mixed leukocyte culture responsiveness by adherent cellsNature, 1976
- Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.The Journal of Experimental Medicine, 1975
- THE ORIGIN OF CELL SURFACE IMMUNOGLOBULIN OF MARROW-DERIVED AND THYMUS-DERIVED LYMPHOCYTES OF THE RATThe Journal of Experimental Medicine, 1974
- Total immunoglobulin of rat thymocytes and thoracic duct lymphocytesEuropean Journal of Immunology, 1974
- The binding of anti‐immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytesEuropean Journal of Immunology, 1974
- CELL SURFACE IMMUNOGLOBULINThe Journal of Experimental Medicine, 1972
- IMMUNOGLOBULIN SPOTS ON THE SURFACE OF RABBIT LYMPHOCYTESThe Journal of Experimental Medicine, 1970
- A study of soluble complexes and uncombined material in antigen-antibody reactions involving allotypic specificities of purified rabbit γ-globulinImmunochemistry, 1965