A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme

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Abstract
A new method suitable for measuring rat liver 3-hydroxy-3-methylglutaryl-CoA reductase [EC 1.1.1.34] activity is described and its advantages over other methods are discussed. An accurate time course was measured for the inhibition of liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol; this enzyme was affected 1 1/4 h after the rats began to consume a cholesterol-rich diet. There was no correlation between concentrations of microsomal cholesterol ester and the activity of 3-hydroxy-3-methylglutaryl-CoA reductase.