Enzymic formation of oestrone 3-glucuronide by rabbit-liver microsomes
- 30 June 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 88 (1), 168-175
- https://doi.org/10.1042/bj0880168
Abstract
Oestrone 3-glucuronide has been identified as the reaction product after incubation of oestrone with a rabbit-liver microsomal preparation, uridine diphosphate glucuronic acid being used as the glucuronic acid donor. The formation of this compound was studied under various experimental conditions. The reaction showed a maximum at pH 8''0-8-2, and Km for oestrone was found to be 9-7 x 10-5[image]. The results are discussed in relation to the general problem of the physiological production of oestrogen glucuronides.Keywords
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