A common epitope between HLA‐B27, ‐B13 and ‐B37 alloantigens defined by a monoclonal antibody
- 1 September 1987
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 30 (3), 97-103
- https://doi.org/10.1111/j.1399-0039.1987.tb01604.x
Abstract
An anti-HLA-B27 monoclonal antibody produced by the hybridoma technique is described. This BD.7 reagent is a cytotoxic IgM antibody. Its reactivity was studied by lymphocytotoxicity tests, indirect immunofluorescence tests and biochemical analysis against an extensive panel of peripheral blood mononuclear cells. All HLA-B27 positive samples, either from normal subjects or from patients with Ankylosing Spondylitis, were recognized by this reagent. Moreover, a cross-reaction was observed with HLA-B13 cells, and a new unexpected reaction with all HLA-B37 cell suspensions. The interest of such a reagent is discussed.Keywords
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