Immunohistochemical localization of the prostaglandin-forming cyclooxygenase in renal cortex
- 1 November 1978
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 235 (5), F451-F457
- https://doi.org/10.1152/ajprenal.1978.235.5.f451
Abstract
An immunohistofluorescence procedure for detecting prostaglandin-forming cyclooxygenase has been used to localize the enzyme in the renal cortex of the cow, guinea pig, rabbit, rat, and sheep. Cyclooxygenase antigenicity was found in endothelial cells lining all arteries and arterioles and in cortical collecting tubules in each species examined. The enzyme was also detected in epithelial cells of Bowman's capsule in the rabbit and in mesangial cells in both ovine and bovine glomerular tufts. That prostaglandins can be formed in renal resistance vessels suggests that it is the synthesis occurring in these vessels which is responsible for the effects of prostaglandins on renal blood flow. Of further note is the correlation that exists between the location of the cyclooxygenase and that of the antidiuretic hormone-responsive adenyl cyclase in the distal nephron.This publication has 11 references indexed in Scilit:
- Demonstration of prostaglandin synthesis in collecting duct cells and other cell types of the rabbit renal medullaProstaglandins, 1977
- Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.JCI Insight, 1977
- Exaggerated prostaglandin biosynthesis and its influence on renal resistance in the isolated hydronephrotic rabbit kidney.JCI Insight, 1977
- Immunochemistry of prostaglandin endoperoxide-forming cyclooxygenases: The detection of the cyclooxygenases in rat, rabbit, and guinea pig kidneys by immunofluorescenceProstaglandins, 1977
- Prostacyclin (PGX) is the endogeneous metabolite responsible for relaxation of coronary arteries induced by arachidonic acidProstaglandins, 1977
- In vivo effect of indomethacin to potentiate the renal medullary cyclic AMP response to vasopressin.JCI Insight, 1977
- Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme.Journal of Biological Chemistry, 1976
- Stimulation of prostaglandin E synthesis in cultured human umbilical vein smooth muscle cells.Proceedings of the National Academy of Sciences, 1976
- The Effect of Indomethacin on Kidney Function and Plasma Renin Activity in ManNephron, 1976
- Micropuncture study of the mammalian urinary concentrating mechanism: evidence for the countercurrent hypothesisAmerican Journal of Physiology-Legacy Content, 1959