Human lymphoblast cell lines: Antibody‐dependent cytotoxicity
- 1 April 1975
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 5 (4), 266-270
- https://doi.org/10.1002/eji.1830050410
Abstract
Five cell lines were isolated after prolonged culture of human buffy coat leukocytes. The cells consisted for the most part of lymphoblasts morphologically similar to those produced by in vivo or in vitro stimulation of small lymphocytes by antigens or mitogens. One of the lines possessed virus-like particles of the Epstein-Barr type. All the established lines were capable of mediating antibody-dependent cytotoxicity (LADC) quantitatively at a level not significantly different from that mediated by freshly isolated, normal human peripheral blood lymphocytes (PBL). The LADC of the lymphoblasts was inhibited by all the human IgG subclasses tested (IgG1, IgG2, IgG3, IgG4) as was the LADC of PBL. It is concluded that, at least so far, the lymphoblasts have retained the properties of the lymphocytes from which they were originally derived as regards their ability to mediate LADC.Keywords
This publication has 18 references indexed in Scilit:
- In vitro Cytodifferentiation of Human Blood LymphocytesDifferentiation, 1974
- THE CYTOTOXIC EFFECT OF MOUSE MACROPHAGES ON SYNGENEIC AND ALLOGENEIC ERYTHROCYTESThe Journal of Experimental Medicine, 1973
- Location of the Proto-Atlantic Suture in the British IslesNature, 1973
- PRODUCTION OF MACROPHAGE MIGRATION INHIBITION FACTOR BY CONTINUOUS CELL LINESThe Journal of Experimental Medicine, 1972
- International Whaling OmissionNature, 1971
- Infectious mononucleosis: immunoglobulin synthesis by cell linesJCI Insight, 1968
- Immunoglobulin Synthesis in vitro by Established Human Cell LinesScience, 1966
- Ultrastructure of Cells Present in Lymph Nodes During the Development of Contact SensitivityInternational Archives of Allergy and Immunology, 1966
- The fine structure of the lymphoid tissue during antibody formationExperimental and Molecular Pathology, 1965
- Contact-Induced Cytotoxicity by Lymphoid Cells Containing Foreign IsoantigensScience, 1965