Comparative study of multidrug resistance evaluated by means of the quantitative immunohistochemical detection of p‐glycoprotein and the functional release of rhodamine 123

Abstract

The immunological detection of P‐Glycoprotein (P‐GP) and the functional release of Rhodamine 123 (R123) have been compared in a number of human and murine cancer cell lines, in chemo‐and/or radiotherapy‐resistant subclones, and in clinical specimens from patients. The chemoresistance level was established from the viability index (IC50) in the presence of doxorubicin. Cytocentrifuge preparations were immunostained with JSB‐1 monoclonal antibody followed by the alkaline phosphatase anti‐alkaline phosphatase technique. The strength of the reaction was quantified by a digital image analyser. The kinetic incorporation and release of Rhodamine 123 were evaluated by flow cytometry. The parent cell lines and radiotherapy resistant subclones showed a low IC50, were JSB‐1 negative and retained R123 during the whole experiment, while the chemoresistant and radio‐chemoresistant cell line mutants had a high IC50, were JSB‐1 positive, and actively pumped the R123 out of the cells. Good correlations were obtained between the IC50, the digital image analysis, and flow cytometry. The kinetic profile of the R123 release allowed the distinction between typical and atypical multidrug resistance phenotypes. These findings were confirmed in clincal specimens from patients. We conclude that antigenic and functional studies are complementary and are useful in experimental and clinical approaches to multidrug resistance.